Device

Part:BBa_K4194015:Design

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


p(TetR)-RBS-EGFP-LVA-RBS-pleD-LVA-terminator-p(Bla)-RBS-(c-di-GMP riboswitch)-mRFP1-LVA-RBS-TetR-LVA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1359
    Illegal NotI site found at 1508
    Illegal NotI site found at 1611
    Illegal NotI site found at 2258
    Illegal NotI site found at 3282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1597
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1811
    Illegal AgeI site found at 3164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2585


Design Notes

Codon optimization for Escherichia coli has been done.


Source

None

References